![]() ![]() Ag NPs are environmentally friendly antibacterial materials and therefore are used in many cosmetic products and medical applications such as hydrogel dressings. It should be noted that depending on the concentration and size silver nanoparticles can be considered as nontoxic as well as toxic. Ĭurrently, many applications of silver NPs are related to their versatile antibacterial activity against a broad spectrum of bacteria without releasing toxic biocides. By exploiting the unique optical and electronic properties of Au NPs, several new methods for ultrasensitive detection of DNA, RNA, and proteins have been developed. Noble metal NPs (in particular Ag NPs) can facilitate important advances in detection, diagnosis, and treatment of human cancers as well as delivery of drug and gene to malignant cells. Applications or potential applications of NPs are not only diverse but also interdisciplinary and are related to material science, electronics, optics, and biomedicine. This is because of the unique properties of nanoscale objects, which are completely different from bulk materials, coatings, and single atoms. Nanoparticles (NPs) of noble metals, especially silver (Ag) and gold (Au) NPs, attract much attention in various fields. The presented results could be extremely important from nanoparticles metrology point of view and should help to understand experimental data especially for the one who works with DLS and/or UV-Vis only. Analysis of the DLS results obtained for polydisperse colloids reveals that several percent of the volume content of bigger NPs could screen completely the presence of smaller ones. Polydisperse colloids were prepared based on the mixture of chemically synthesized monodisperse colloids well characterized by atomic force microscopy (AFM), transmission electron microscopy (TEM), DLS, and UV-Vis spectroscopy. The size and size distribution of artificially prepared polydisperse silver nanoparticles (NPs) colloids were studied using dynamic light scattering (DLS) and ultraviolet-visible (UV-Vis) spectroscopy. Although the theory and mathematical basics of DLS technique are already well known, little has been done to determine its limits experimentally. Sample polydispersity can distort the results, and we could not see the real populations of particles because big particles presented in the sample can screen smaller ones. This method can be used for measurements of narrow particle size distributions especially in the range of 2–500 nm. Dynamic light scattering is a method that depends on the interaction of light with particles. ![]()
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